Celiac disease is known as gluten enteropathy, caused by damaging of small intestinal\nepithelium cells following gluten consumption. Gluten is classified in two families of\nglutenin and gliadin. Gliadin is divided into Ã?±, Ã?³ and Ãâ?° groups. One of the most effective\nmethods to rectify or minimize gene expression is RNAi technology. The present\nstudy has been conducted to the structure of Ã?±-gliadin gene to produce proprietary\nRNAi cassette for silencing wheat Ã?± -gliadin gene. So nucleotide sequence of\nÃ?±-gliadin gene involved in design for PCR amplification by online primer-blast software.\nThe amplification was extracted from agarose gel and ligated to pTG19 cloning\nvector. After cloning of the recombinant plasmid in E. coli , they were sequenced.\nThen they were used for construction of specific and efficient RNAi cassette. The\nproduced vector by this strategy is considered as an effective step in developing genetically\nengineered gluten-less or low content gluten wheat.
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